AFDye 430 Alkyne
Abs/Em Maxima: 430/537 nm
Extinction Coefficient: 15,000
Spectrally Similar Dyes: Alexa Fluor? 430, CF? 430
AZDye? 430 Alkyne (Alexa Fluor? 430 Alkyne equivalent) is a green-fluorescent alkyne-activated probe routinely used for imaging of moderate to high abundance azide-containing biomolecules. AZDye? 430 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
A probe for copper-less azide detection (AZDye? 430 DBCO) is also available for application where the presence of copper is not acceptable.
AZDye? 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.
AZDye? 430 is structurally identical to Alexa Fluor? 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor? 430 and CF?430 Dye.
For application where the presence of copper is not acceptable, please consider our AZDye? 430 DBCO probe for copper-less detection of azide-modified molecules.
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor? 350, CF? 350, DyLight 350, AMCAAZDye? 350 is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click react…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405AZDye? 405 Azide is a water-soluble, pH-insensiti…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor? 350, CF? 350, DyLight 350, AMCAAZDye? 350 Alkyne (Alexa Fluor? 350 Alkyne equivalent) is a blue-fluorescent, alkyne-activated probe that reacts with azid…
Abs/Em Maxima: 430/537 nmExtinction Coefficient: 15,000Spectrally Similar Dyes: Alexa Fluor? 430, CF? 430AZDye? 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click re…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor? 350, CF? 350, DyLight 350, AMCAAZDye? 350 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not re…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405AZDye? 405 Alkyne reacts with azides via a copper…
Abs/Em Maxima: 494/517 nmExtinction Coefficient: 73.000Flow Cytometry Laser Line: 488 nmMicroscopy Laser Line: 488 nmSpectrally Similar Dyes: Fluorescein, Alexa Fluor? 488, CF? 488A, DyLight? 488, Atto? 488AZDye? 488 Azide (Alexa Fluor? 488 Azi…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405ZDye? 405 DBCO reacts with azides via a copper-fr…
