AFDye 555 DBCO
Abs/Em Maxima: 555/572 nm
Extinction Coefficient: 155,000
Flow Cytometry Laser Line: 532 nm or 555 nm
Microscopy Laser Line: 633 nm or 647 nm
Spectrally Similar Dyes: Alexa Fluor? 555, CF? 555, DyLight? 549, Cy3 Dye
AZDye? 555 DBCO is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye? 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
AZDye? 555 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. AZDye? 555 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye? 555 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AZDye? 555 is structurally similar to Alexa Fluor? 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor? 555, CF? 555 Dye, or any other Cyanine3 based fluorescent dyes. AZDye? 555 DBCO can be used a less expensive alternative to Alexa Fluor? 555 sDIBO.
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor? 350, CF? 350, DyLight 350, AMCAAZDye? 350 is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click react…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405AZDye? 405 Azide is a water-soluble, pH-insensiti…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor? 350, CF? 350, DyLight 350, AMCAAZDye? 350 Alkyne (Alexa Fluor? 350 Alkyne equivalent) is a blue-fluorescent, alkyne-activated probe that reacts with azid…
Abs/Em Maxima: 430/537 nmExtinction Coefficient: 15,000Spectrally Similar Dyes: Alexa Fluor? 430, CF? 430AZDye? 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click re…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor? 350, CF? 350, DyLight 350, AMCAAZDye? 350 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not re…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405AZDye? 405 Alkyne reacts with azides via a copper…
Abs/Em Maxima: 494/517 nmExtinction Coefficient: 73.000Flow Cytometry Laser Line: 488 nmMicroscopy Laser Line: 488 nmSpectrally Similar Dyes: Fluorescein, Alexa Fluor? 488, CF? 488A, DyLight? 488, Atto? 488AZDye? 488 Azide (Alexa Fluor? 488 Azi…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405ZDye? 405 DBCO reacts with azides via a copper-fr…
