PC DBCO-NHS Ester
Photocleavable DBCO-NHS ester reagent contains an amine reactive group linked to DBCO moiety through a spacer arm containing a photocleavable moiety. Molecules jointed by the copper-free click reaction can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).
1.Perdue, J., et al. (2020). Optical Control of Cytokine Signaling via Bioinspired, Polymer-Induced Latency. Biomacromolecules., 21(7), 2635-44. [PubMed]
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor? 350, CF? 350, DyLight 350, AMCAAZDye? 350 is a blue-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click react…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405AZDye? 405 Azide is a water-soluble, pH-insensiti…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor? 350, CF? 350, DyLight 350, AMCAAZDye? 350 Alkyne (Alexa Fluor? 350 Alkyne equivalent) is a blue-fluorescent, alkyne-activated probe that reacts with azid…
Abs/Em Maxima: 430/537 nmExtinction Coefficient: 15,000Spectrally Similar Dyes: Alexa Fluor? 430, CF? 430AZDye? 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click re…
Abs/Em Maxima: 346/445 nmExtinction Coefficient: 19,000Spectrally Similar Dyes: Alexa Fluor? 350, CF? 350, DyLight 350, AMCAAZDye? 350 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not re…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405AZDye? 405 Alkyne reacts with azides via a copper…
Abs/Em Maxima: 494/517 nmExtinction Coefficient: 73.000Flow Cytometry Laser Line: 488 nmMicroscopy Laser Line: 488 nmSpectrally Similar Dyes: Fluorescein, Alexa Fluor? 488, CF? 488A, DyLight? 488, Atto? 488AZDye? 488 Azide (Alexa Fluor? 488 Azi…
Abs/Em Maxima: 402/424 nmExtinction Coefficient: 35,000Flow Cytometry Laser Line: 405 nmMicroscopy Laser Line: 405 nmSpectrally Similar Dyes: Alexa Fluor? 405, CF? 405, Cascade Blue?, DyLight? 405ZDye? 405 DBCO reacts with azides via a copper-fr…
