Metabolic Labeling of Glycoproteins 下載為PDFGeneral Information This protocol details the preparation of cell lysates metabolically labeled with azido- or alkynylsugar. However, investigators may wish to determine the optimal concentration of metabolic reagent as well as labeling time individually for metabolic labeling for…

Metabolic Labeling with AHA or HPG 下載為PDFGeneral Information For imaging, culture cells on cover slips or other desired imaging-compatible surface, approximately 1 × 106 cells are sufficient for one detection. 1 × 106 cells are required to prepare 100 μg of lysate. Metabolic Labeling of Cells The following is a standa…